molecular and serological evaluation of toxoplasma gondii infection in reared turkeys in fars province, iran

background toxoplasmosis is a parasitic disease caused by the protozoan toxoplasma gondii. this parasite infects most of warm-blooded animals, including birds. turkeys are one of these animals which might be infected by this parasite. little is known about the prevalence of t. gondii in turkeys in iran. objectives the current study aimed to evaluate the rate of toxoplasma infection in turkeys in fars province, southern iran. materials and methods sera and tissues (brain, neck and tongue) of 54 turkeys were collected from shiraz slaughterhouse in fars province. anti-toxoplasma antibodies were assessed in the collected sera using modified agglutination test (mat), while tissues were evaluated by polymerase chain reaction (pcr) and bioassay methods. results t. gondii antibodies (mat titer: ≥ 1:40) were found in 89.8% of turkeys. t. gondii dna was detected in 61.6% of turkey tissues and brain had the highest rate of infection. brain tissues from each animal were bioassayed and toxoplasma tissue cysts were found in 11.5% and toxoplasma dna in 62% of inoculated mice. conclusions results of this study validated a relatively high level of toxoplasma infection in reared turkeys and turkey meat might be considered as an infection sources for human.